Coding

Part:BBa_K1124004:Design

Designed by: Yuta Otsuka   Group: iGEM13_UT-Tokyo   (2013-09-11)

cI repressor (+LVA, -barcode, E234K)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

barcode was deleted by site-directed mutagenesis.

E233K mutatagenesis.

The template for PCR was BBa_C0051


Source

BBa_C0051

References